WebExpand Long Template PCR System is an enzyme mix that contains thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. This … WebExpand Long Template PCR System is an enzyme mix that contains thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. This …
Expand Long Template System 11681842001 MERCK THIRD PA…
WebFeb 1, 2024 · 3.7. Formulation of a final PCR protocol. A PCR protocol was finalized based on the experimental results for the amplification of lengthy GC-rich DNA sequences from the M. bovis genome. The protocol involved the use of PrimeSTAR GXL enzyme, DMSO at 5%, and a master mix as described in Materials and Methods, Section 2.4. WebSep 26, 2024 · The clones with desired mutations can be first identified by a restriction enzyme digest prior to sequence verification. ... and 3 units of high-fidelity PCR enzyme mix (Expand Long Template PCR System, Roche) in a final volume of 25 μl. The PCR cycles were initiated at 95 °C for 10 min to denature the template DNA, followed by 14 ... chamblee post office po box
Expand™ Long Template PCR System Sigma-Aldrich
http://web.as.uky.edu/biology/faculty/mirabito/BIO%20510%20Fall%202407/Expand%20Long%20Template.pdf WebPrimeSTAR GXL is a hot-start, high-fidelity enzyme that is optimized to amplify GC-rich templates, samples with excess templates, and long PCR products up to 30 kb. PrimeSTAR GXL Premix enables you to simplify your amplification workflow, saving you time and reducing potential errors. Simply add primers and template, and you are ready … WebUse this enzyme blend with its threefold greater accuracy than Taq Polymerase for more precise amplification of longer DNA templates. Maximize target yield. Minimize … chamble share price