Diacylglycerol kinase atp
WebGoto and Kondo (1993) isolated rat brain cDNAs encoding a novel 90-kD DGK. The predicted 90-kD DGK protein was 58% identical to the rat 80-kD DGK. Both proteins contain EF-hand motifs, cysteine-rich zinc finger-like sequences, and putative ATP-binding sites. Northern blot analysis revealed that the 90-kD DGK was expressed as an approximately … WebA putative diacylglycerol kinase from bacillus anthracis str. Sterne: Source: Bacillus anthracis. Organism_taxid: 260799. Strain: sterne. Gene: bak_5150. Expressed in: escherichia coli. Expression_system_taxid: 511693. Chains: A, B, C, D (293 residues) CATH domains: 3.40.50.10330 2.60.200.40
Diacylglycerol kinase atp
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WebElucidation of an ATP binding motif unique for lipid kinases • Identification of ligand binding sites for developing DGKα-selective inhibitors Summary … WebThe main clinical disorder of glycerol metabolism that has been described is glycerol kinase deficiency. Glycerol kinase is encoded by the GK gene on chromosome Xp21.2; thus, in distinction to all of the fatty acid oxidation defects, it is an X-linked rather than autosomal disorder. Symptoms are due to the inability for glycerol to enter the …
WebNov 5, 2015 · Decrease of AMPK-related signal transduction and insufficient lipid oxidation contributes to the pathogenesis of obesity and type 2 diabetes. Previously, we identified that diacylglycerol kinase-δ (DGKδ), an enzyme involved in triglyceride biosynthesis, is reduced in skeletal muscle from type 2 diabetic patients. WebRNA polymerase II (also called RNAP II and Pol II) is an enzyme found in eukaryotic cells. It catalyzes the transcription of DNA to synthesize precursors of mRNA and most snRNA and microRNA. In humans, RNAP II consists of seventeen protein molecules (gene products encoded by POLR2A-L, where the proteins synthesized from POLR2C, POLR2E, and …
WebDeoxycytidine Kinase [D08.811.913.696.620.175] Diacylglycerol Kinase [D08.811.913.696.620.200] Fructokinases [D08.811.913.696.620.225] Galactokinase [D08.811.913.696.620.240] Glucokinase [D08.811.913.696.620.250] Glycerol Kinase [D08.811.913.696.620.275] Hexokinase [D08.811.913.696.620.300] Kanamycin Kinase … WebThe Saccharomyces cerevisiae DGK1 gene encodes a diacylglycerol kinase enzyme that catalyzes the formation of phosphatidate from diacylglycerol. Unlike the diacylglycerol kinases from bacteria, plants, and animals, the yeast enzyme utilizes CTP, instead of ATP, as the phosphate donor in the reaction. Dgk1p contains a CTP transferase domain that is …
WebDiacylglycerol kinases (DGKs) are a family of enzymes that catalyze the ATP-dependent phosphorylation of diacylglycerol (DAG) to phosphatidic acid (PtdOH). Despite the critical role in lipid biosynthesis, both DAG and PtdOH have been shown as bioactive lipids mediating a number of signaling pathways.
WebDiacylglycerol kinase catalyzes direct ATP-to-diacylglycerol phosphoryl transfer via a random equilibrium kinetic mechanism at a modest rate that nevertheless roughly matches the rate at which diacylglycerol can flip-flop across the bacterial membrane from its periplasmic site of production to diffuse to the cytosolically-disposed DAGK active site. fm40 fimco sprayerWeb3.13.2.2.3.4 Diacylglycerol kinase. DGK from E. coli is a small integral membrane protein with 121 residues that catalyzes the conversion of diacylglycerol and ATP to phosphatidic acid. The protein functions as a homotrimer, with each monomer containing three TM α helices and two amphipathic α helices found at the membrane surface. greensboro day school logoWeb萤火虫荧光素酶mRNA（ARCA，5-moUTP）表达的萤光素酶蛋白，最初是从萤火虫Photinus pyralis中提取的。. 该酶催化ATP依赖性D-荧光素氧化以产生氧化荧光素，氧化荧光素是一种单重激发的化合物，当返回其基态时发光。. 萤火虫荧光素酶是一种常用的生物发光报告基因 ... fm 407 texasWebFeb 1, 2007 · The diacylglycerol kinase inhibitor R59949 markedly reduced the cellular PA content, suggesting the diacylglycerol kinase activity was involved in a large part of the PA production in HEK293 cells. greensboro day school jobsWebTo determine if p47-phox kinase activity was increased in pre-eclamptic B-LCLs a non-radioactive kinase assay was developed to measure p47-phox kinase activity in whole cell lysates. In resting cells a basal p47-phox kinase activity could be measured, which could be rapidly enhanced by the addition of PMA directly into the kinase assay. greensboro day school calendar 2022Diacylglycerol kinase (DGK or DAGK) is a family of enzymes that catalyzes the conversion of diacylglycerol (DAG) to phosphatidic acid (PA), utilizing ATP as a source of the phosphate. In non-stimulated cells, DGK activity is low, allowing DAG to be used for glycerophospholipid biosynthesis, but on receptor activation of the phosphoinositide pathway, DGK activity increases, driving the conversio… greensboro day school photosWebMar 9, 2024 · Title: Diacylglycerol kinase θ counteracts protein kinase C-mediated inactivation of the EGF receptor. DGK-theta; directly interacts with, and is activated by, basic proteins such as histone H1 and Tau with nm affinity, consistent with a potential role for a polybasic protein or protein domain in the activation of this enzyme. greensboro day school camp