Ctll 2 proliferation assay

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August undefined, 2024

WebApr 14, 2024 · Human IL-2 as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells (orange, green, red, respectively). 6'6 3$*(5HFRPELQDQW+XPDQ,/ KÉ 3URWHLQ6'6 3$*( 2 μg/lane of Recombinant Human ILKÉ2 Protein (Catalog # BT-002) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and … WebCTLL-2 is a clone of cytotoxic T cells derived from a C57BL/6 mouse. The cells are dependent upon Interleukin-2 (IL-2) for growth and can be used to assay for IL-2. …

CTLL-2 93042610 - Sigma-Aldrich

WebNov 29, 2013 · Most bioassays measured the proliferative effect of IL-2 on the murine cytotoxic T cell-line, CTLL-2 or the HT-2 cell-line but employed different readouts for assessing the proliferation (Gillis et al., 1978, Gieni et al., 1995). In one laboratory, however, the human T cell-line, KIT 225 was used (Hori et al., 1987). WebBiological Activity: The ED50 as determined in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells is less than 0.5 ng/ml. Sequence APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHF DPRDVVSNIN VFVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT. eloy jimenez 2021 stats

A convenient and reliable IL-2 bioassay using frozen CTLL-2 to …

WebDownload scientific diagram CTLL-2 cells proliferation assay of purified superagonist to evaluate its bioactivity. The EC 50 was calculated by the four-parameter fit logistic equation to be 30 ... WebCTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. Culture Medium RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human … WebSupport protocols are provided for the quantitation of CTLL-2, CT4.S, or CT.h4S proliferation using a standard [3H]thymidine incorporation method or by using the 3-(4,5 … tee vs atar

Life-extended glycosylated IL-2 promotes Treg induction …

WebCTLL-2 cells were seeded in a 96-well plate and treated with varying concentrations of Human Recombinant IL-2. Cell proliferation was measured using a colorimetric assay. In the graph below, the specific activity was determined to be ≥ 5 x … http://immunservice.com/wp-content/uploads/User-Manual-WST-1-CTLL-2-WST1.CTLL150813-EN-V4.pdf tee 배관 규격WebJun 2, 2012 · A bioassay for IL-2 using CTLL-2 cells. Supernatants of tumor cells (PCl-13) transduced with the gene for human IL-2 were collected and tested for the ability to … eloy jimenez spring training

"WebWhen maintained in continuous culture, however, the overall sensitivity of this cell line is highly variable. To overcome this problem, large batches of CTLL-2 were grown, frozen and tested for their ability to detect low levels of IL-2 (0.01 IU) in dose response assays. Batches meeting this criterion were stored in liquid nitrogen until required. " - Ctll 2 proliferation assay

Ctll 2 proliferation assay

Measurement of human and murine interleukin 2 and interleukin 4

WebThe present invention relates to cancer immunotherapy. In particular, provided herein are fusion proteins for targeting tumor associated macrophages with immunostimulatory agents. WebThe purified siIL-15 stimulates the proliferation of cytotoxic T lymphocytes line (CTLL)-2 and Kit 225 cells with EC50 values of 3.1 and 32.5 ng/mL, respectively.

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WebThe ED 50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10 7 IU/mg. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 mm filtered concentrated solution in 20 mM PB, pH3.5, with 5 % ...

WebApr 11, 2024 · Recombinant Mouse IL2 (Catalog # 402 ML) stimulates cell proliferation of the CTLL 2 mouse cytotoxic T cell line. The ED 50 for this effect is 0.1 0.4 ng/mL. 6'6 3$*(1 μg/lane of Recombinant Mouse IL2 was resolved with SDSPAGE under reducing (R) conditions and visualized by silver staining, WebApr 13, 2024 · of Recombinant Human IL-2 as measured in a cell proliferation assay CTLL 2 mouse cytotoxic T cells (orange, green, red, respectively). 6'6 3$*(5HFRPELQDQW+XPDQ,/ 3URWHLQ6'6 3$*( 1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver …

http://www.wrenshallab.com/uploads/5/6/3/8/56385051/mttctll2.pdf Webfrom the Mo7e and CTLL-2 cyto-kine-dependent proliferation assay, PFC-1 exhibited an approx-imately 10-fold stronger potency than rhIL-15 in PBMC prolifera-tion, while 10 nM rhIL-15 led to a proliferation rate of 20.71% and 1 nM PFC-1 resulted in a mean value of 22.05% (Fig. 2B). The enhanced activity could be due to the higher activity of IL ...

WebMay 24, 2015 · Cell Proliferation Assay Application of a colorimetric CTLL-2 cell proliferation assay for the evaluation of IL-15 antagonists Authors: Yunier Rodríguez Center for Genetic Engineering and...

WebThe biological activity of the recovered soluble rhIL-2 protein was determined in a proliferation assay using CTLL-2 cells. Human IL-2 purchased from Sigma-Aldrich Co. … elou jean goubaldWebCTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. 培地 RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL … tee 追加WebThe bioactivity of the superagonist was characterized by CTLL-2 cells proliferation assay in vitro, showing its capability of stimulating the expansion of memory CD8 + T cells (cluster of differentiation) in mouse spleen. tee 英語WebFeb 16, 2024 · CCK-8 assay TGF-β could inhibit the IL-2 dependent proliferation of CTLL-2 and HT-2. We performed CCK-8 assays to measure the capability of YM101 antagonizing the effect of TGF-β. CTLL-2 and HT-2 cells (1 × 10 3 per well) were seeded in 96-well plates (3599, Corning). Then, TGF-β1 and antibodies (10 5 pM) were added into plates. eloy jimenez milb statsWebApplication CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. [ 1] [ 2] Culture Medium RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS). Subculture Routine Maintain cultures between 3-9 x 10 5 cells/ml; 5% CO 2; 37°C. elp u404Web2. Add WST-1 reagent from CTLL-2 cell proliferation Kit 3. Incubate for 1 – 4 h 4. Measure absorbance at 420 – 480 nm 5. Analyze data Advantages at a glance: Easy to use o … tee-ei lustigWebApr 5, 2024 · measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 0.0300-0.250 ng/mL. Two independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of Animal-FreeTM Recombinant Human IL-2. %LRDFWLYLW\ tee-1 timer